The Confederation of African Football (CAF) held the draw on Tuesday at its headquarters in Cairo with the attendance of Ahly Football Director Sayed Abdel-Hafiz and Zamalek assistant coach Usama Nabih.
Group A matches will be started with Zamalek and Ahly derby on 19, 20 or 21 July while the away match will be held on 13, 14 or 15 September.
Titleholders Ahly reached the group stage after winning 2-1 at home to Tunisia's Bizertin, while their bitter-rivals Zamalek qualified after a 3-3 aggregate draw with Ethiopia's Saint George.
The two Cairo giants were paired together last year with TP Mazembe of DR Congo and Ghana's Berekum Chelsea in which Ahly and Mazembe qualified.
Egyptian football has been facing extraordinary circumstances since the Port Said disaster forced the suspension of domestic competitions for over a year, but Egyptian clubs and national teams are having success in continental competitions.
Ismaily and ENPPI are set to play Tunisia's C.A. Bizertin and Saint George of Ethiopia respectively in the CAF Confederation Cup round of 16. They will be in different groups if they qualified, according to Tuesday's draw.
The national team are leading Group G of the World Cup qualifiers, five points clear of second-placed Guinea.
The juniors are preparing for the World Cup in Turkey after winning the African tournament.
CAF Champions League group stage draw:
Ahly, Zamalek, AC Leopards of Congo, Orlando Pirates of South Africa
Group B: Esperance, Coton Sport of Cameroon, Recreativo Libolo of Angola, Sewe San Pedro of Ivory Coast
The CAF Confederation Cup Group stage draw:
Group A St. George (ETH) vs Enppi (EGY) St Malien (MLI) vs Lydia (BUR) C.S.S. (TUN)/ Rangers (NGA) JSM Bejaia (ALG) vs ESS (TUN)
Group B TP Mazembe (RDC)/ Liga M. (MOZ) CAB (TUN) vs Ismaily (EGY) ES Sétif (ALG) vs US Bitam (GAB) FUS (MOR) vs ASFAR (MOR)